o c phosphate buffered saline pbs (Thermo Fisher)

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Thermo Fisher o c phosphate buffered saline pbs
O C Phosphate Buffered Saline Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+c/PHOSPHATE+BUFFERED+SALINE+PBS/pmc13108891-48-29-34
Average 99 stars, based on 1 article reviews

o c phosphate buffered saline pbs - by Bioz Stars, 2026-10

99/100 stars

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Recombinant:

Article Title: HDAC6 Inhibition Reduces Seeded Tau and α-Synuclein Pathologies in Primary Neuron Cultures and Wild-type Mice
Article Snippet: .. 355 Recombinant mouse tau PFFs prepared as previously described (Crowe et al., 2013) 356 were used as standards and were prepared in a two-fold dilution series from 320 ng/ml 357 JN uro sci Ac ce pte M an us cri p 16 to 20 pg/ml in PBS plus 0.4% BSA. mTau8 was also biotinylated (ThermoFisher Scientific) 358 for use as detection antibody, with the antibody diluted to 0.125 μg/mL in Buffer C (20 359 mM sodium phosphate, pH 7.0, 2 mM EDTA, 400 mM sodium chloride, 1% BSA and 360 0.005% Thimerosal (ThermoFisher Scientific). ..

Purification:

Article Title: Molecular mechanism of action of small molecule SMT-738 on bacterial lipoprotein transporter LolCDE.
Article Snippet: .. LolA was purified by a HisTrap HP column (GE Healthcare) in buffer C (20 mM Tris-HCl, pH 7.8, 300 mM NaCl) containing 300 mM imidazole. followed by additional purification using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer B. Cryo-electron microscopy sample preparation and data acquisition For cryo-EM sample preparation, 3 μl aliquots of purified LolCDE-SMT-738 complex (1 mg/ml) were applied to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 300 mesh copper). .. Following 3 s blotting at 8 °C and 100% humidity, the grid was plunge-frozen in liquid ethane cooled with liquid nitrogen using a Vitrobot Mark IV system (Thermo Fisher Scientific).

Article Title: Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance
Article Snippet: .. The purification of Yl Ku70/80 complex was performed as in the case of Pyl1p, with the following modifications: (i) The plasmid pDuet-Ku70/80 was transformed into One Shot BL21(DE3) cells; (ii) the thawed cells were resuspended in buffer C [20 mM Tris-HCl pH 7.5, 150 mM NaCl] containing 1 mg/ml lysozyme (Sigma-Aldrich), 1x protease inhibitors cocktail cOmplete (Roche), 10 mM MgCl 2 (Sigma-Aldrich), 2 μg/ml RNase A (Invitrogen) and 1 U/ml DNase I (Applichem); (iii) the protein extract was mixed with 0.2 ml cobalt resin (Sigma-Aldrich) equilibrated with buffer C containing 0.25% (v/v) Triton X-100 and incubated for 3 h end-over-end at 4°C. .. The beads were then washed three times with buffer C containing 0.1% (v/v) Triton X-100) and four times with buffer D [50 mM HEPES-NaOH (pH 7.2), 150 mM NaCl, 1 mM DTT, 10% glycerol]; (iv) the beads were resuspended in 1 ml of buffer D containing PreScission protease (GE27-0843-01, Cytiva) and incubated 3 h end-over-end at 4°C.

Article Title: Structural basis and pathological implications of the dimeric OS9-SEL1L-HRD1 ERAD Core Complex
Article Snippet: Proteins were eluted in 5 CV buffer B supplemented by 0.1 mg/mL 3xFLAG peptide (APExBIO). .. The eluate was concentrated and further purified by size-exclusion chromatography (SEC) (Superose 6 Increase, 10/300 GL column, Cytiva) in buffer C (20 mM HEPES, 150 mM NaCl, 0.02% glyco-diosgenin/GDN, pH7.5) for cryo-EM. ..

Cryo-Electron Microscopy:

Sample Prep:

Ligation:

Article Title: A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplification
Article Snippet: BtsI (R0707S, New England Biolabs) according to the manufacturer’s protocol and stored at −20°C until use. .. One nM ssDNA M13 (N4040S, New England Biolabs), dsDNA supercoiled or nicked M13 DNA, serving as ligation targets, were incubated with 10 nM padlock probes, 0.8 U/μl Taq ligase and a biotin-conjugated RCA primer 2 (30 nM) at 55°C for 2 h. The reactions were diluted 200-fold with buffer C (5 mM Tris-HCl pH 7.5, 0.5 mM EDTA (15575020, Thermo Fisher), 1 M NaCl and 0.05% Tween20) and added to streptavidin-coated slides (custom-made by TRIDIA) with FlexWell incubation chambers (204916, Grace Bio-Labs) for 1 h at room temperature. .. The slides were rinsed twice with buffer C, followed by addition of 250 μM dNTP, 0.2 U/μl phi29 DNA polymerase and 15% PEG4000 (supplemented with T4 DNA ligase (EL0012, Thermo Fisher)) in 1 × phi29 buffer for RCA at 37°C for 1.5 h. After two washes, RCA products were stained for 10 min at 37°C with 25 nM Alexa Fluor-594-conjugated detection 3 in buffer C, supplemented with 10% dextran sulfate (S4030, Millipore).

Incubation:

Size-exclusion Chromatography:

Article Title: Structural basis and pathological implications of the dimeric OS9-SEL1L-HRD1 ERAD Core Complex.
Article Snippet: Nature Communications | (2026) 17:2064 10 3xFLAG peptide (APExBIO). .. The eluate was concentrated and further purifiedby size-exclusion chromatography (SEC) (Superose6 Increase, 10/300 GL column, Cytiva) in buffer C (20mM HEPES, 150mM NaCl, 0.02% glyco-diosgenin/GDN, pH7.5) for cryo-EM. ..

Centrifugation:

Article Title: Shining light on drug discovery: optogenetic screening for TopBP1 biomolecular condensate inhibitors
Article Snippet: The cell pellet was suspended in 5× packed cell volume of hypotonic buffer A (10 mM Hepes–KOH, pH 7.9, 10 mM KCl, 1.5 mM MgCl 2 , 0.5 mM dithiothreitol (DTT), and 0.5 mM Phenylmethylsulfonyl fluoride (PMSF)), supplemented with a cocktail of protease inhibitors (cOmplete, ethylenediaminetetraacetic acid (EDTA) free; Roche) and phosphatase inhibitors (Thermo Fisher Scientific), and incubated on ice for 5 min. Next, the cells were spun down at 500 × g for 5 min, suspended in 2× packed cell volume of buffer A, and lysed by Dounce homogenization using a tight-fitting pestle. .. Nuclei were collected by centrifugation at 4000 × g for 5 min at 4°C and extracted in one nuclei pellet volume of buffer C (20 mM Hepes–KOH, pH 7.9, 600 mM KCl, 1.5 mM MgCl 2 , 0.2 mM EDTA, 25% glycerol, 0.5 mM DTT, and 0.5 mM PMSF) supplemented with cocktails of protease and phosphatase inhibitors, and mixed on a rotating wheel at 4°C for 30 min. Nuclear extracts (supernatants) were recovered by centrifugation (16 000 × g for 15 min at 4°C) and dialyzed using Slide-A-Lyzer Dialysis Cassettes (3500-Da protein molecular weight cutoff; Thermo Fisher Scientific) against buffer D (20 mM Hepes–KOH, pH 7.9, 100 mM KCl, 0.2 mM EDTA, 20% glycerol, 0.5 mM DTT, and 0.5 mM PMSF). ..

Molecular Weight:

Protease Inhibitor:

Article Title: Risk reshapes amygdala representation of choice
Article Snippet: .. Samples were processed in triplicate, such that bilateral punches from 3 mice were acquired, stored in ice-cold Buffer B, then transferred to an ice-cold Teflon/glass homogenizer (details) containing ∼1 ml of Buffer C (10 mM HEPES, 150 mM KCl, 5 mM MgCl2, 0.5 mM DL-Dithiothreitol (DTT), 80 U/ml RNasin Plus Ribonuclease Inhibitor (Promega), 40 U/ml SUPERase-In RNase Inhibitor (Invitrogen, Waltham, MA, USA), 100 μg/ml cycloheximide, protease inhibitor cocktail (Roche cOmplete, Sigma-Aldrich, St. Louis, MO, USA). .. Tissue in Buffer C were hand-homogenized on ice (10 strokes), followed by high-speed homogenization at ∼750 rpm (Glas-Col).

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